Chlorophyll a Estimates (MELEE) - 1

*This chlorophyll a data is from the MELEE study (Microbial Ecology of the Lake Erie Ecosystem) and was not funded by the IFYLE study.*

Size-fractionated chlorophyll-a is determined immediately after sample collection from parallel filtration of samples collected on 0.2-µm, 2-µm and 20-µm nominal pore-size polycarbonate filters (47 mm dia.; Osmonics) after extraction (ca 24 h, 4 °C) in 90 % acetone. Chlorophyll-a retained on the different size class filters is quantified using the non-acidification protocol (Welschmeyer 1994) with either a TD-700 or AU-10 fluorometer (both from Turner Designs).

Contact Info

Steven W. Wilhelm, PhD
Department of Microbiology
The University of Tennessee
Knoxville, TN
37996-0845
phone: 865-974-0665 labs:865-974-0682, 865-974-4014
fax: 865-974-4007
email: